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| Registros recuperados: 190 | |
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| Laus,J.L.; Ortiz,J.P.D.; Vicenti,F.A.M.; Brito,F.L.C.; Wehrle,L.M.; Castro Netto,A.; Lisbão,C.B.S.. |
| Relata-se o caso de um bovino com, aproximadamente, seis horas de vida, apresentando histórico de febre, apatia, inapetência e desconforto ocular. No exame oftálmico rotineiro, encontraram-se valores do teste da lágrima de Schirmer aumentados, diminuição da pressão intra-ocular, hemorragia conjuntival, uveíte anterior, edema corneal e injeção ciliar em ambos os olhos. Coletou-se amostra de sangue para realização de esfregaço sangüíneo, hemograma e bioquímica sérica. No esfregaço visibilizaram-se corpúsculos de Anaplasma marginale em hemácias parasitadas. Quanto à bioquímica sérica, não foram observadas alterações. Os mesmos exames foram realizados na mãe do animal tendo como resultado a positividade para A. marginale. Para o controle da uveíte foi... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Holstein; Uveitis; Anaplasmosis. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600038 |
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| Santos,Rodrigo Marques dos; Barreta,Marcos Henrique; Frajblat,Marcel; Cucco,Diego Córdova; Mezzalira,Joana Claudia; Bunn,Silvério; Cruz,Fabiano Buss; Vieira,Arnaldo Diniz; Mezzalira,Alceu. |
| The objective of this study was to determine the effects of vacuum-cooled liquid nitrogen on the development of vitrified immature (germinal vesicle stage; GV) and mature (metaphase II; MII) bovine oocytes after re-warming. Liquid nitrogen was exposed to either atmospheric pressure or to a vacuum (300mm Hg for 45sec); the latter decreased the temperature of the liquid nitrogen to -200°C. Partially denuded oocytes were vitrified either just after selection (GV) or after 22 hours of in vitro maturation (MII) in TCM 199 medium + 10% of estrous mare serum. For vitrification, oocytes were firstly exposed to an intermediate solution (10% EG + 10% DMSO) for 30sec, followed by the vitrification solution (20% EG + 20% DMSO + 0.5M sucrose) for 20sec. Groups of three... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vitrification; Cryopreservation; Oocytes; Bovine; Vacuum. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000500024 |
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| Silva-Zacarias,Francielle Gibson da; Alfieri,Amauri Alcindo; Spohr,Kledir Anderson Hofstaetter; Lima,Bruna Azevedo de Carvalho; Negrão,Fábio Juliano; Lunardi,Michele; Freitas,Julio Cesar de. |
| Chlamydophila abortus (C. abortus) is associated with reproductive problems in cattle, sheep, and goats. Diagnosis of C. abortus using embryonated chicken eggs or immortalized cell lines has a very low sensitivity. Polymerase chain reaction (PCR) assays have been used to detect C. abortus infection in clinical specimens and organ fragments, such as placenta, fetal organs, vaginal secretions, and semen. The aim of this study was to develop a PCR assay for the amplification of an 856-bp fragment of the rRNA gene of the Chlamydiaceae family. The PCR assay was evaluated using organs from 15 mice experimentally infected with the S26/3 reference strain of C. abortus. The results of the rRNA PCR were compared to the results from another PCR system (Omp2 PCR) that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Ovine; Caprine; Reproductive failures; Chlamydophila abortus; PCR. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700014 |
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| Baldassi,L.; Barbosa,M.L.; Bach,E.E.; Iaria,S.T.. |
| Ten out of eighty-nine strains biochemically identified as Clostridium perfringens, isolated from bovine organs, were selected by their different results showed in toxigenicity test on mice. Those and the standard strains, ATCC types A, B, C, and D, had their virulence exalted through serial intramuscular inoculation into guinea pigs. Results showed that, for toxigenic strains (6), one or two passages were enough to cause exaltation, while for the atoxigenic (4), five or six inoculations were needed. Esterase electrophoresis of standard and isolated strains, with and without exaltation, was performed. Electrophoresis analysis permits the following conclusions: strains that do not show any clinical symptoms in mice, when exalted demonstrate decreased... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium perfringens; Bovine; Electrophoresis; Virulence exaltation. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300007 |
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| Mezzalira,Alceu; Viera,Arnaldo Diniz; Barbieri,Dilmar Paulo; Machado,Mariana Fernandes; Thaler Neto,André; Bernadi,Mari Lourdes; Silva,Carlos Antônio Mondino; Rubin,Mara Iolanda Batistella. |
| In this study, the influence of cytochalasin B on the survival and development after vitrification of oocytes and in vitro produced (IVP) bovine blastocysts was evaluated. In the Experiment I, 956 oocytes were matured for 22h and were immediately vitrified (Vitri treatment) or exposed for 15-20 minutes, to 7.5µg/ml (CB7.5Vitri treatment) or 45µg/ml (CB45Vitri treatment) cytochalasin B solutions, before vitrification. After 30 seconds of exposure to SV1 [400µl TCM-Hepes with 10% fetal serum (SF), 50µl ethylene glycol (EG) and 50µl DMSO], and 20 seconds to SV2 (300µl trehalose 1,0M + 20% SF, 100µl EG and 100µl DMSO) solutions, the oocytes were vitrified in Open Pulled Straws (OPS). The rewarming was performed at 37-38ºC in two steps of 5 minutes each, into... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Cytochalasin B; Embryos. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000500008 |
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| Nozaki, Hirofumi; Miyamoto, Akio; Hayashi, Ken-Go; Matsui, Motozumi; Yoshida, Takashi; Nakamura, Tadashi; Arai, Ikichi; Urashima, Tadasu; 宮本, 明夫; 松井, 基純; 中村, 正; 浦島, 匡. |
| The activity of α3-D-mannoside-β-1,2-N-acetylglucosaminyltransferase I (GnT I; EC 2.4.1.101), which catalyzes the first step in the conversion of oligomannose to complex or hybrid N-glycans of glycoproteins, was detected in bovine follicular fluid (bFF). The GnT I activity in bFF had a pH optimum of 5.8 and an absolute requirement for either Co2+, Mn2+, or Mg2+, the activity being stimulated by these cations in the above order. The apparent Km value for α1-3α1-6 mannopentaose of GnT I in bFF was 2.17 mM. The substrate specificity for the GnT I activity decreased in the following order: α1-3α1-6 mannopentaose> α1-3α1-6 mannotriose>α1-3 mannobiose. The GnT I activity in bFF from large atretic follicles was significantly higher than in that from large... |
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Palavras-chave: N-acetylglucosaminyltransferase I; Dominant follicle; Atretic follicle; Follicular fluid; Bovine. |
| Ano: 2004 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4185 |
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| 岸本, 海織; 住谷, 峻; 李, 奇子; 清水, 純一郎; 山川, 和宏; 滄木, 孝弘; 羽田, 真悟; 松本, 高太郎; 石井, 三都夫; 佐々木, 直樹; 猪熊, 壽; 山田, 一孝; KISHIMOTO, Miori; SUMIYA, Takashi; LEE, Ki-Ja; SHIMIZU, Junichiro; YAMAKAWA, Kazuhiro; AOKI, Takahiro; HANEDA, Shingo; MATSUMOTO, Kotaro; ISHII, Mitsuo; SASAKI, Naoki; INOKUMA, Hisashi; YAMADA, Kazutaka. |
| 牛臨床におけるComputed Radiography (CR) の有用性を確立するため，管球-カセッテ間距離およびImaging Plate (IP) の読み取り時間が画質に与える影響について検討した.管球-カセッテ間距離は画質の視覚的スコア(粒状性，鮮鋭性)に影響しなかった. IPの読み取り時間は画質の鮮鋭性と相関しなかったが，粒状性と相関が認められた.しかし読み取り時聞が12時間以内の場合には問題にならない程度であると考えられた.管球-力セッテ間距離を再現よく保つことが難しく， IP読み取り時間のばらつきが想定される牛の臨床画像診断においてCRは有用なX線撮影システムであると考えられた. The purpose of this study was to investigate the effect of X-ray tube-cassette distance and image scanning time on image quality to establish the benefit of Computed Radiography (CR) in bovine clinical practice. The tube-cassette distance had no effect on the visual evaluation score (graininess and sharpness). The image scanning time correlated with graininess but not sharpness. From these results，it was concluded that accidental variations in the tube-cassette... |
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Palavras-chave: 牛; Computed Radiography (CR); フェーデイング; Imaging Plate (IP); ポータブルX線発生装置; Bovine; Computed Radiography (CR); Fading; Imaging Plate (lP); Portable x-ray generator. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2939 |
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| Registros recuperados: 190 | |
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